Anti-phosphopeptide Antibodies

Anti-phosphorylated peptide antibodies have become very useful reagents in the study of cell signal transduction. Specific antibody probes that are able to identify which of the many proteins in the signal transduction path are phosphorylated or have been de-phosphorylated help in the understanding of cell regulation, growth and apoptosis.

Mimotopes is a world leader in the generation of affinity-enhanced anti-phosphopeptide antisera. Mimotopes obtains affinity enhanced anti-peptide antibodies from rabbits immunized with a carrier protein-peptide conjugate. Affinity enhancement is performed to remove antibodies specific to the non-phosphorylated version of the peptide. An understanding that other specific anti-peptide antibodies, not involving the phosphorylated residues, may be generated is crucial to how the phosphorylated peptide antisera are used, and to the interpretation of results obtained using these antibodies. The table below shows examples of antiserum with enhanced specificity for the phosphorylated peptide sequence.